Human Measles Virus (MV) IgM ELISA Kit

This ELISA kit uses Capture-ELISA as the method to qualitatively detect the Measles Virus-IgM (MV- IgM) in human serum and plasma. The ELISA Microtiter plate provided in this kit has been pre-coated with Mouse-anti-human IgM (μ chain). Samples are added to the ELISA Microtiter plate wells and the IgM antibody in which will be captured. Free components are washed away (including the specific IgG antibody). The MV antigen and anti-MV-antibody-HRP conjugated reagent are added to each well and incubate. The “anti-μ chain- MV-IgM antibody- MV antigen-HRP conjugated antibody” compound will form if the sample contains MV-IgM. Free components are washed away. The TMB substrate is added to initiate the color developing reaction and form a blue product. The presence of MV-IgM can be determined according to the OD value after colorimetric assay with the Micro-plate reader.