ENR(Enrofloxacin) ELISA Kit

Representative Elabscience ELISA kit packaging; contents vary by catalogue and size

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Manufactured by:Elabscience
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ENR(Enrofloxacin) ELISA Kit

ENR(Enrofloxacin) ELISA Kit from Elabscience.

SKU:
ABM150289-1
Manufacturer SKU
E-FS-E159
Product type
ELISA Kits
TargetENR
ApplicationsELISA
Species / reactivityUniversal
Sample typeTissues, raw milk, reconstituted milk, bovine serum, eggs, cooked food (meat products), pig urine, feed

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ENR(Enrofloxacin) ELISA Kit is supplied by Elabscience as an ELISA kit for quantitative analysis. Supplier-listed sample types: Tissues, raw milk, reconstituted milk, bovine serum, eggs, cooked food (meat products), pig urine, feed.

Enrofloxacin is a third-generation quinolone antibacterial drug, also known as ethyl ciprofloxacin. It has obtained U.S. FDA approval on October 4, 1996, dedicated to livestock and aquatic products. It can bind to the bacterial DNA gyrase A subunit, thereby inhibiting the enzyme's cutting and joining functions, preventing bacterial DNA replication, and exhibiting antibacterial effects.

Detection method: Colorimetric method;ELISA;Competitive. Supplier-listed detection limit: 1 ppb; 2 ppb; 5 ppb; 10 ppb; 20 ppb; 30 ppb; 100 ppb (matrix-dependent). Incubation time: 45 minutes. Format: Microtiter plate with 96 wells (12 strips with 8 wells each).

Available pack sizes: 96T / 96T*3 / 96T*5. Refer to the manufacturer instructions for sample preparation and assay conditions. Research use only.

Test principle

This kit uses Competitive-ELISA as the method forthe quantitative detection. Itcan detect Enrofloxacin (ENR) in samples, such as muscle, milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate inthis kit has been pre-coated with coupled antigen. During the reaction, ENR in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-ENR antibody. Then Horseradish Peroxidase (HRP) conjugate isadded toeach microtiter plate well, and substrate reagent is added for color development. There isa negative correlation between the OD value of samples and the concentration of ENR. The concentration of ENR in the samples can be calculated by comparing the OD of the samples to the standard curve.
Key facts & specifications
Research areas
Metabolism
Also known as
Enrofloxacin
Assay type
ELISA
Detection method
Colorimetric method;ELISA;Competitive
Sensitivity
1 ppb; 2 ppb; 5 ppb; 10 ppb; 20 ppb; 30 ppb; 100 ppb (matrix-dependent)
Detection range
See the manual for details
Plate format
Microtiter plate with 96 wells (12 strips with 8 wells each).
Wells / tests
96T / 96T*3 / 96T*5
Assay duration
45 minutes
Form / buffer
ELISA kit
Quantity per pack
96T / 96T*3 / 96T*5
Technical note
Matrix-specific supplier sensitivity / detection limits: Tissue Method 1 (fish, shrimp)---approx. 1 ppb; Tissue Method 1 (chicken, duck, pork, beef, mutton)---approx. 2 ppb; Tissue Method 2 (chicken, duck)---approx. 5 ppb; Tissue Method 2 (pork, beef, mutton), raw milk, reconstituted milk, bovine serum, eggs---approx. 10 ppb; cooked food (meat products)---approx. 20 ppb; porcine urine, Tissue Method 3 (pork liver, fish, shrimp)---approx. 30 ppb; feed---approx. 100 ppb. Detection instrument: Microtiter plate spectrophotometer (450 nm/630nm) Principle source: https://789.bio/ea/LxvzGdJg#page=2 Lead-time discrepancy: supplied price list states 2-3 business days; manufacturer variant records state 4-6 days. Confirm dispatch timing when ordering.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Evidence status by application and model.

EvidenceTissuesraw milkreconstituted milkbovine serumeggscooked food (meat products)pig urinefeed
ABMIUM Verified
Identifiers & database references
Manufacturer SKU
E-FS-E159
Storage, stability & shipping
Shelf life
12 months from production; follow the labelled expiry date
Storage conditions
2-8℃, avoid freeze, 11-12 months
Short-term storage
2-8℃, avoid freeze, 11-12 months
Long-term storage
2-8℃, avoid freeze, 11-12 months
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£227.00