Anatine Avian Influenza Virus Antibodies ELISA Kit

This kit uses the blocking ELISA method for the detection of anatine avian influenza virus antibodies in the serum of anatine. anatine avian influenza antigen is pre-coated on the micro-wells of the microtiter plate strips. During the test,diluted test serum is added and incubated. If the sample contains anatine avian influenza antigen-specific antibodies, they will bind to the antigen coated on the plate. After washing to remove unbound antibodies and other components, enzyme-labeled anti-anatine avian influenza antibody is added. The antibodies in the sample block the binding of the enzyme-labeled antibody to the coated antigen. After washing to remove unbound enzyme conjugate, TMB substrate solution is added to the wells. The blue signal produced by enzymatic catalysis is inversely proportional to the antibody content in the sample. After adding stop solution to terminate the reaction, the absorbance (OD value) of the reaction wells is measured at 450 nm using a microplate reader.