Ratten-ACSL4(Acyl-CoA Synthetase Long Chain Family Member 4) ELISA Kit von ELK Biotechnology
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Das Ratten-ACSL4 (Acyl-CoA Synthetase Long Chain Family Member 4) ELISA Kit ist ein ELK Biotechnology ELISA Kit zur Forschungsmessung von ACSL4.
Das in diesem Kit angewandte Testprinzip ist der Sandwich-Enzymimmunoassay. Die in diesem Kit enthaltene Mikrotiterplatte wurde mit einem spezifischen Antikörper gegen Ratten-ACSL4 vorbeschichtet. Standards oder Proben werden zu den entsprechenden Mikrotiterplattenvertiefungen gegeben, dann mit einem Biotin-konjugierten Antikörper, der spezifisch für Ratten-ACSL4 ist. Als Nächstes wird mit Meerrettichperoxidase (HRP) konjugiertes Avidin zu jeder Mikrotiterplattenvertiefung gegeben und inkubiert. Nach Zugabe der TMB-Substratlösung zeigen nur die Vertiefungen, die Ratten-ACSL4, Biotin-konjugierten Antikörper und Enzym-konjugiertes Avidin enthalten, eine Farbänderung. Die Enzym-Substrat-Reaktion wird durch Zugabe von Schwefelsäurelösung beendet und die Farbänderung wird spektrophotometrisch bei einer Wellenlänge von 450 nm ± 10 nm gemessen. Die Konzentration von Ratten-ACSL4 in den Proben wird dann durch Vergleich der OD der Proben mit der Standardkurve bestimmt.
Alternative Namen sind ACS4; FACL4; LACS4; MRX63; MRX68; Fatty-Acid-Coenzyme A Ligase,Long-Chain 4; Mental Retardation,X-Linked 63; Lignoceroyl-CoA Synthase.
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Gewebehomogenate | Zelllysate | Zellkulturüberstände | Andere biologische Flüssigkeiten |
|---|---|---|---|---|---|---|
| Musterkompatibilität |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Acyl-CoA synthetase long chain family member 4 (ACSL4), also known as FACL4 and long-chain-fatty-acid--CoA ligase 4, activates long-chain fatty acids to acyl-CoA esters. Human ACSL4 maps to NCBI Gene 2182 and reviewed UniProtKB O60488; this record links the verified target to related ABMIUM catalogue products.
Biological function: ACSL4 catalyses activation of long-chain fatty acids to fatty acyl-CoA esters for cellular lipid synthesis and fatty-acid degradation. NCBI and reviewed UniProt describe a preference for arachidonate; UniProt also identifies ACSL4 as a ferroptosis activator by generating activated polyunsaturated-fatty-acid substrates that can undergo lipid peroxidation.
