Rat VEGF ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK0540 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Rat |
| Tested applications | ELISA |
| Gene name | VEGFA |
| Protein name | Vascular endothelial growth factor A |
| Sample type | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
| Assay range | 15.6 pg/ml - 1,000 pg/ml |
| Sensitivity | <1 pg/ml |
| Specificity | Natural and recombinant rat VEGF |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A27-R190 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P16612 |
| NCBI Gene ID | 83785 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | Vascular endothelial growth factor A |
| Also known as | L VEGFA; MVCD1; Vascular permeability factor; VEGF; VEGF A; VEGFA; VPF |
| Species reactivity | Rat |
| Purity | Natural and recombinant rat VEGF |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <1 pg/ml |
| Detection range | 15.6 pg/ml - 1,000 pg/ml |
| Sample types |
cell culture supernatantsserum and plasma (heparinEDTAcitrate).
See sample validation ›
|
Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum and plasma (heparin | |||||
| EDTA | |||||
| citrate). |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.