ELISA Kit supplied by Boster Bio Read more ›
Rat TNF Alpha/Tumor necrosis factor High Sensitivity ELISA Kit PicoKine® is a research-use ELISA kit from Boster Bio for measuring Tumor necrosis factor in Cell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA). samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
Rat TNF Alpha/Tumor necrosis factor High Sensitivity ELISA Kit PicoKine® (96 Tests). Quantitate Rat Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. The standard product used in this kit is recombinant rat TNFalpha, consisting of 157 amino acids with the molecular mass of 17KDa.
| Collaborator | Boster Bio |
| Partner model | HSEK0526 |
| Target | TNF |
| Reactivity | Rat |
| Applications | ELISA |
| Cross-reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: L80-L235 |
| Sample types | Cell culture supernatant,Cell lysates,serum and plasma (heparin,EDTA). |
| Detection range | 1.56 pg/ml - 100 pg/ml |
| Sensitivity | 0.006pg/ml |
| Purity | Natural and recombinant rat TNF alpha |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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