Rat PDGF-AB ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK0485 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Rat |
| Tested applications | ELISA |
| Gene name | PDGFB, PDGFA |
| Sample type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay range | 31.2 pg/ml - 2,000 pg/ml |
| Sensitivity | <3 pg/ml |
| Specificity | Natural and recombinant rat PDGF-AB |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182) |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | Q05028/P28576 |
| NCBI Gene ID | 24628, 25266 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | PDGFB, PDGFA |
| Also known as | PDGF A chain; _x000D_PDGF B chain; _x000D_PDGF1; _x000D__x000D__x000D_PDGF2; _x000D_PDGFA; _x000D_PDGFB; _x000D_Platelet derived growth factor AB heterodimer |
| Species reactivity | Rat |
| Purity | Natural and recombinant rat PDGF-AB |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <3 pg/ml |
| Detection range | 31.2 pg/ml - 2,000 pg/ml |
| Sample types |
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
See sample validation ›
|
Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| cell lysates | |||||
| serum and plasma (heparin | |||||
| EDTA). |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.