ELISA Kit supplied by Boster Bio Read more ›
Rat PDGF-AB ELISA Kit PicoKine® is a research-use ELISA kit from Boster Bio for measuring PDGFB, PDGFA in Cell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA). samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
Rat PDGF-AB ELISA Kit PicoKine® (96 Tests). Quantitate Rat PDGF-AB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.The platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains. The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281. The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner. PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23. The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22. The standard product used in this kit is recombinant rat PDGF-AB (A chain Ser87-Arg196; B chain Ser74-Thr182) with the molecular mass of 24.8KDa.
| Collaborator | Boster Bio |
| Partner model | EK0485 |
| Target | PDGFB, PDGFA |
| Reactivity | Rat |
| Applications | ELISA |
| Cross-reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: (R86-K204) + (S74-T182) |
| Sample types | Cell culture supernatant,Cell lysates,serum and plasma (heparin,EDTA). |
| Detection range | 31.2 pg/ml - 2,000 pg/ml |
| Sensitivity | <3 pg/ml |
| Purity | Natural and recombinant rat PDGF-AB |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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