Rat IL-12(p70) ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK1652 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Rat |
| Tested applications | ELISA |
| Gene name | Il12a |
| Protein name | Interleukin-12 subunit alpha |
| Sample type | cell culture supernatants and serum. |
| Assay range | 15.6 pg/ml - 1,000 pg/ml |
| Sensitivity | <1.5 pg/ml |
| Specificity | Natural and recombinant rat IL-12(P70) |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: (M23 - S335) + (R23 - S215) |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | Q9R103 |
| NCBI Gene ID | 84405 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | Interleukin-12 subunit alpha |
| Also known as | CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL12; IL-12; IL12A; IL-12A; Interleukin 12 subunit alpha; NFSK; NKSF1; P35 |
| Species reactivity | Rat |
| Purity | Natural and recombinant rat IL-12(P70) |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <1.5 pg/ml |
| Detection range | 15.6 pg/ml - 1,000 pg/ml |
| Sample types |
cell culture supernatants and serum.
See sample validation ›
|
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants and serum. |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.