Rat Endostatin PicoKine® Quick ELISA Kit
ELISA Kit supplied by Boster Bio Read more ›
Product Description
Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | FEK1377 |
| Product category | Quick ELISA Kits |
| Reactive species | Rat |
| Tested applications | ELISA |
| Gene name | Col18a1 |
| Protein name | Collagen type XVIII, alpha |
| Sample type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/ml - 10,000 pg/ml |
| Sensitivity | <15 pg/ml |
| Specificity | Natural and recombinant rat Col18a1 |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H1591-K1774 |
| Storage | Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.) |
| UniProt | Q9WUW5 |
| NCBI Gene ID | 85251 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | Collagen type XVIII, alpha |
| Also known as | COL18A1; Collagen alpha 1(XVIII) chain; collagen; type XVIII; alpha 1; Endostatin; KNO; KNO1 |
| Species reactivity | Rat |
| Purity | Natural and recombinant rat Col18a1 |
| Shelf life | Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.) |
| Sensitivity | <15 pg/ml |
| Detection range | 156 pg/ml - 10,000 pg/ml |
| Sample types |
cell culture supernatantscell lysatesserum and plasma (heparinEDTA)
See sample validation ›
|
Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| cell lysates | |||||
| serum and plasma (heparin | |||||
| EDTA) |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.