ELISA Kit supplied by Boster Bio Read more ›
Rat Endostatin PicoKine® Quick ELISA Kit is a research-use ELISA kit from Boster Bio for measuring Collagen type XVIII, alpha in Cell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA) samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
Rat Endostatin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
| Collaborator | Boster Bio |
| Partner model | FEK1377 |
| Target | Collagen type XVIII, alpha |
| Reactivity | Rat |
| Applications | ELISA |
| Cross-reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H1591-K1774 |
| Sample types | Cell culture supernatant,Cell lysates,serum and plasma (heparin,EDTA) |
| Detection range | 156 pg/ml - 10,000 pg/ml |
| Sensitivity | <15 pg/ml |
| Purity | Natural and recombinant rat Col18a1 |
| Shelf life | Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.) |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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