Rat BDNF ELISA Kit EZ-Set™ (DIY Antibody Pairs)
ELISA Antibody Pair supplied by Boster Bio Read more ›
Product Description
Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EZ0308 |
| Product category | EZ-Set™ ELISA Kits (Ab Pairs) |
| Reactive species | Rat |
| Tested applications | ELISA |
| Gene name | BDNF |
| Protein name | Neurotrophic factor BDNF precursor form |
| Sample type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate). |
| Assay range | 31.2 pg/ml - 2,000 pg/ml |
| Specificity | Natural and recombinant rat BDNF |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: H129-R247 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P23363 |
| NCBI Gene ID | 24225 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 5 plates/kit |
Key Facts
| Target | Neurotrophic factor BDNF precursor form |
| Also known as | Abrineurin; BDNF |
| Species reactivity | Rat |
| Purity | Natural and recombinant rat BDNF |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Detection range | 31.2 pg/ml - 2,000 pg/ml |
| Sample types |
cell culture supernatantscell lysatesserum and plasma (heparinEDTAcitrate).
See sample validation ›
|
Sample Type Validation
5 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| cell lysates | |||||
| serum and plasma (heparin | |||||
| EDTA | |||||
| citrate). |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.