Mouse TREM-1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Mouse TREM-1 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse TREM1 in cell culture supernatants, lysates, tissue, serum and plasma (heparin or EDTA).

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by Trem1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EZ0845
Product category	EZ-Set™ ELISA Kits (Ab Pairs)
Reactive species	Mouse
Tested applications	ELISA
Gene name	TREM1
Protein name	Triggering receptor expressed on myeloid cells 1
Sample type	cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
Assay range	31.2 pg/ml - 2,000 pg/ml
Specificity	Natural and recombinant mouse TREM-1
Immunogen	Expression system for standard: NS0; Immunogen sequence: A21-S202
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	Q9JKE2
NCBI Gene ID	58217

Available variants

Conjugation	Size
Not applicable	5 plates/kit