Mouse TNF Alpha/Tumor necrosis factor ELISA Kit EZ-Set™ (DIY Antibody Pairs)
ELISA Antibody Pair supplied by Boster Bio Read more ›
Product Description
Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EZ0527 |
| Product category | EZ-Set™ ELISA Kits (Ab Pairs) |
| Reactive species | Mouse |
| Tested applications | ELISA |
| Gene name | Tnf |
| Protein name | Tumor necrosis factor |
| Sample type | cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 15.6 pg/ml - 1,000 pg/ml |
| Specificity | Natural and recombinant mouse Tnf |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: L80-L235 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P06804 |
| NCBI Gene ID | 21926 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 5 plates/kit |
Key Facts
| Target | Tumor necrosis factor |
| Also known as | Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-a; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor; tnfa; tnfα |
| Species reactivity | Mouse |
| Purity | Natural and recombinant mouse Tnf |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Detection range | 15.6 pg/ml - 1,000 pg/ml |
| Sample types |
cell culture supernatantsserum and plasma (heparinEDTA)
See sample validation ›
|
Sample Type Validation
3 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum and plasma (heparin | |||||
| EDTA) |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.