Mouse SDF-1/CXCL12 ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
SDF-1 (stromal cell-derived factor-1) is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). This gene is located on chromosome 10q11.1. SDF-1 is produced in two forms, SDF-1alpha/CXCL12a and SDF-1beta/CXCL12b, by alternate splicing of the same gene. Chemokines are characterized by the presence of four conserved cysteines, which form two disulfide bonds. The CXCL12 proteins belong to the group of CXC chemokines, whose initial pair of cysteines are separated by one intervening amino acid. CXCL12 is strongly chemotactic for lymphocytes. CXCL12 was shown to be expressed in many tissues in mice (including brain, thymus, heart, lung, liver, kidney, spleen and bone marrow). CXCL12 is a highly efficacious lymphocyte chemoattractant. In addition, CXCL12 induces intracellular actin polymerization in lymphocytes. CXCL12 is a substrate for the matrix metalloproteinase-2, which cleaves an CXCL12 N-terminal tetrapeptide. The standard product used in this kit is recombinant SDF-1 with the molecular mass of 8Kda.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK0500 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Mouse |
| Tested applications | ELISA |
| Gene name | CXCL12 |
| Protein name | Stromal cell-derived factor 1 |
| Sample type | cell culture supernatants, serum and plasma (heparin) |
| Assay range | 62.5 pg/ml - 4,000 pg/ml |
| Sensitivity | <10 pg/ml |
| Specificity | Natural and recombinant mouse Cxcl12 |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: K22-M93 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P40224 |
| NCBI Gene ID | 20315 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | Stromal cell-derived factor 1 |
| Also known as | Cxcl12; Sdf1; Stromal cell-derived factor 1; SDF-1; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; TPAR1; C-X-C motif chemokine 12; Pre-B cell growth-stimulating factor; PBSF; Thymic lymphoma cell-stimulating factor; TLSF |
| Species reactivity | Mouse |
| Purity | Natural and recombinant mouse Cxcl12 |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <10 pg/ml |
| Detection range | 62.5 pg/ml - 4,000 pg/ml |
| Sample types |
cell culture supernatantsserum and plasma (heparin)
See sample validation ›
|
Sample Type Validation
2 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum and plasma (heparin) |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.