Mouse OPN / Osteopontin ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK0483 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Mouse |
| Tested applications | ELISA |
| Gene name | SPP1 |
| Protein name | Osteopontin |
| Sample type | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
| Assay range | 156 pg/ml - 10,000 pg/ml |
| Sensitivity | <10 pg/ml |
| Specificity | Natural and recombinant mouse OPN |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: L17-N294 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P10923 |
| NCBI Gene ID | 20750 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | Osteopontin |
| Also known as | BNSP; _x000D_Bone sialoprotein 1; _x000D_Bone sialoprotein I; _x000D__x000D__x000D_BSPI; _x000D_Early T lymphocyte activation 1; _x000D_ETA 1; _x000D_MGC110940; _x000D_Nephropontin; _x000D_OPN; _x000D_Osteopontin; _x000D_OSTP_HUMAN; _x000D_Secreted phosphoprotein 1; _x000D_Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); _x000D_Secreted phosphoprotein 1 (osteopontin bone sialoprotein); _x000D_SPP-1; _x000D_SPP1; _x000D_Urinary stone protein; _x000D_Uropontin |
| Species reactivity | Mouse |
| Purity | Natural and recombinant mouse OPN |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <10 pg/ml |
| Detection range | 156 pg/ml - 10,000 pg/ml |
| Sample types |
cell culture supernatantsserumplasma (heparinEDTA) and urine.
See sample validation ›
|
Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum | |||||
| plasma (heparin | |||||
| EDTA) and urine. |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.