ELISA Antibody Pair supplied by Boster Bio Read more ›
Mouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) is a research-use antibody from Boster Bio for studying Interleukin-12 subunit alpha in ELISA workflows with listed reactivity for Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Mouse IL-12(p70) ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse IL-12(p70) in cell culture supernatants and serum.Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
| Collaborator | Boster Bio |
| Partner model | EZ0422 |
| Target | Interleukin-12 subunit alpha |
| Reactivity | Mouse |
| Applications | ELISA |
| Cross-reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: (M23-S335) + (R23-A215) |
| Sample types | cell culture supernatants and serum |
| Detection range | 15.6 pg/ml - 1,000 pg/ml |
| Purity | Natural and recombinant mouse IL-12(p70) |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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