ELISA Kit supplied by Boster Bio Read more ›
Mouse GM-CSF ELISA Kit PicoKine® is a research-use ELISA kit from Boster Bio for measuring Granulocyte-macrophage colony-stimulating factor in Cell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA). samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
Mouse GM-CSF ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Csf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa.
| Collaborator | Boster Bio |
| Partner model | EK0365 |
| Target | Granulocyte-macrophage colony-stimulating factor |
| Reactivity | Mouse |
| Applications | ELISA |
| Cross-reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: A18-K141 |
| Sample types | Cell culture supernatant,Cell lysates,serum and plasma (heparin,EDTA). |
| Detection range | 15.6 pg/ml - 1,000 pg/ml |
| Sensitivity | <1 pg/ml |
| Purity | Natural and recombinant mouse GM-CSF |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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