Mouse CHIT1 ELISA Kit PicoKine®
ELISA Kit supplied by Boster Bio Read more ›
Product Description
Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1; 1 E4. It is distinct from another member known as acidic mammalian chitinase (AMC), encoded by the CHIA gene, in several aspects. AMC/CHIA is expressed mainly in alveolar macrophages and in both the mouse and human gastrointestinal tract. CHIT1, however, is expressed exclusively by phagocytes in humans and in the gastrointestinal tract, tongue, fore-stomach, and Paneth cells of the small intestine in mice. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pH and can be processed into a C-terminally truncated 39 kDa form. Human CHIT1 is the best of three biomarkers in the monitoring of Gaucher disease. The other two commonly used markers include acid phosphatase and angiotensin-converting enzyme. Human CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK2033 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Mouse |
| Tested applications | ELISA |
| Gene name | Chit1 |
| Protein name | Chitotriosidase-1 |
| Sample type | cell culture supernatants, serum and plasma (heparin, EDTA). |
| Assay range | 31.2 pg/ml - 2,000 pg/ml |
| Sensitivity | <15 pg/ml |
| Specificity | Natural and recombinant mouse CHIT1 |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: A22-S464 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | Q9D7Q1 |
| NCBI Gene ID | 71884 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
| Target | Chitotriosidase-1 |
| Also known as | CHI3; CHIT; CHIT1; Chitinase 1; chitinase 1 (chitotriosidase); Chitotriosidase 1 |
| Species reactivity | Mouse |
| Purity | Natural and recombinant mouse CHIT1 |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <15 pg/ml |
| Detection range | 31.2 pg/ml - 2,000 pg/ml |
| Sample types |
cell culture supernatantsserum and plasma (heparinEDTA).
See sample validation ›
|
Sample Type Validation
3 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum and plasma (heparin | |||||
| EDTA). |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.