Mouse CD73/NT5E ELISA Kit PicoKine®

Mouse CD73/NT5E ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Nt5e in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK2131
Product category	PicoKine® ELISA Kits
Reactive species	Mouse
Tested applications	ELISA
Gene name	Nt5e
Protein name	5'-nucleotidase
Sample type	cell culture supernatants, serum and plasma (heparin, EDTA)
Assay range	0.156 ng/ml - 10 ng/ml
Sensitivity	<20 pg/ml
Specificity	Natural and recombinant mouse Nt5e
Immunogen	Expression system for standard: CHO; Immunogen sequence: W29-S551
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	Q61503
NCBI Gene ID	23959

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.