Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Mouse CCL20/MIP-3 alpha ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse CCL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EZ0454
Product category	EZ-Set™ ELISA Kits (Ab Pairs)
Reactive species	Mouse
Tested applications	ELISA
Gene name	CCL20
Protein name	C-C motif chemokine 20
Sample type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay range	7.8 pg/ml - 500 pg/ml
Specificity	Natural and recombinant mouse CCL20/MIP-3 alpha
Immunogen	Expression system for standard: E.coli; Immunogen sequence: A28-M97
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	O89093
NCBI Gene ID	20297

Available variants

Conjugation	Size
Not applicable	5 plates/kit