Human Pan Trypsin ELISA Kit PicoKine®

Human Pan Trypsin ELISA Kit PicoKine® (96 Tests). Quantitate Human Pan Trypsin in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK1531
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	Pan Trypsin
Protein name	Trypsin-2
Sample type	cell culture supernatants, serum and plasma (heparin).
Assay range	31.2 pg/ml - 2,000 pg/ml
Sensitivity	<10 pg/ml
Specificity	Natural and recombinant human Pan Trypsin
Immunogen	Expression system for standard: NS0; Immunogen sequence: A16-S247
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P07478
NCBI Gene ID	5645

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.