Human Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®

Human Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine® (96 Tests). Quantitate Human ELANE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

In humans, neutrophil elastase is encoded by the ELANE gene, which resides on chromosome 19. It is mapped to 19p13.3. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK2064
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	ELANE
Protein name	Neutrophil elastase
Sample type	cell culture supernatants, serum and plasma (heparin, EDTA).
Assay range	0.78 ng/ml - 50 ng/ml
Sensitivity	<50 pg/ml
Specificity	Natural and recombinant human ELANE
Immunogen	Expression system for standard: NS0; Immunogen sequence: I30-H267
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P08246
NCBI Gene ID	1991

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.