ELISA Kit ABMIUM Verified™Collaborator Tested
ABM-32782

Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

ABMIUM Verified™|Collaborator Tested|Boster Bio

ELISA Kit supplied by Boster Bio Read more ›

Related formats
Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine® Boster Bio
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Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine® Boster Bio

Product Description

Human Nesfatin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NUCB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference EK1138
Product category PicoKine® ELISA Kits
Reactive species Human
Tested applications ELISA
Gene name NUCB2
Protein name Nucleobindin-2
Sample type cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay range 31.2 pg/ml - 2,000 pg/ml
Sensitivity <10 pg/ml
Specificity Natural and recombinant mouse CCL8
Immunogen Expression system for standard: E.coli; Immunogen sequence: V25-L106
Storage Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt P80303
NCBI Gene ID 4925

Available variants

Conjugation Size
Not applicable 96 wells/kit, with removable strips.

Key Facts

Product type ELISA Kit Target: Nucleobindin-2
Target Nucleobindin-2
Also known as DNA binding protein NEFA; Gastric cancer antigen Zg4; NEFA; NUCB2; nucleobindin 2
Species reactivity Human
Purity Natural and recombinant mouse CCL8
Shelf life Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Sensitivity <10 pg/ml
Detection range 31.2 pg/ml - 2,000 pg/ml
Sample types
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
See sample validation ›
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Sample Type Validation

4 sample types
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
cell culture supernatants
cell lysates
serum and plasma (heparin
EDTA).

Specifications

Target
Nucleobindin-2
Also known as
DNA binding protein NEFA; Gastric cancer antigen Zg4; NEFA; NUCB2; nucleobindin 2
Purity
Natural and recombinant mouse CCL8
Sensitivity
<10 pg/ml
Detection range
31.2 pg/ml - 2,000 pg/ml
Sample types
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
Species reactivity
Human

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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