Human Leptin ELISA Kit PicoKine®

Human Leptin ELISA Kit PicoKine® (96 Tests). Quantitate Human LEP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK0437
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	LEP
Protein name	Leptin
Sample type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay range	62.5 pg/ml - 4,000 pg/ml
Sensitivity	<10 pg/ml
Specificity	Natural and recombinant human Leptin
Immunogen	Expression system for standard: E.coli; Immunogen sequence: V22-C167
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P41159
NCBI Gene ID	3952

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.