ELISA Antibody Pair supplied by Boster Bio Read more ›
Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs) is a research-use antibody from Boster Bio for studying Leptin in ELISA workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LEP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.
| Collaborator | Boster Bio |
| Partner model | EZ0437 |
| Target | Leptin |
| Reactivity | Human |
| Applications | ELISA |
| Cross-reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V22-C167 |
| Sample types | Cell culture supernatant,Cell lysates,serum and plasma (heparin,EDTA). |
| Detection range | 62.5 pg/ml - 4,000 pg/ml |
| Purity | Natural and recombinant human Leptin |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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