Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs)
ELISA Antibody Pair supplied by Boster Bio Read more ›
Product Description
Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EZ0437 |
| Product category | EZ-Set™ ELISA Kits (Ab Pairs) |
| Reactive species | Human |
| Tested applications | ELISA |
| Gene name | LEP |
| Protein name | Leptin |
| Sample type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay range | 62.5 pg/ml - 4,000 pg/ml |
| Specificity | Natural and recombinant human Leptin |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V22-C167 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | A4D0Y8 |
| NCBI Gene ID | 3952 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 5 plates/kit |
Key Facts
| Target | Leptin |
| Also known as | LEP; leptin; OB; Obese protein; Obesity factor; OBS |
| Species reactivity | Human |
| Purity | Natural and recombinant human Leptin |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Detection range | 62.5 pg/ml - 4,000 pg/ml |
| Sample types |
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
See sample validation ›
|
Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| cell lysates | |||||
| serum and plasma (heparin | |||||
| EDTA). |
Specifications
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.