ELISA Antibody Pair ABMIUM Verified™Collaborator Tested
ABM-31959

Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

ABMIUM Verified™|Collaborator Tested|Boster Bio

ELISA Antibody Pair supplied by Boster Bio Read more ›

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Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Boster Bio
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Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) Boster Bio

Product Description

Human IL-8 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference EZ0413
Product category EZ-Set™ ELISA Kits (Ab Pairs)
Reactive species Human
Tested applications ELISA
Gene name CXCL8
Protein name Interleukin-8
Sample type cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay range 15.6 pg/ml - 1,000 pg/ml (cell culture supernates) 7.8 pg/ml - 500 pg/ml (serum, plasma)
Specificity Natural and recombinant human IL-8
Immunogen Expression system for standard: E.coli; Immunogen sequence: S28-S99
Storage Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt P10145
NCBI Gene ID 3576

Available variants

Conjugation Size
Not applicable 5 plates/kit

Key Facts

Product type ELISA Antibody Pair Target: Interleukin-8
Target Interleukin-8
Also known as C X C motif chemokine 8; CXCL8; CXCL8; IL8; CXCL8/IL-8; Emoctakin; GCP 1; GCP1; IL 8; IL 8(1 77); IL8; IL-8; IL8/NAP1 form I Interleukin 8; IL8/NAP1 form II; IL8/NAP1 form VI; interleukin 8; MDNCF; MDNCF b IL 8(5 77) IL 8(6 77); NAF; NAF IL 8(7 77); NAP 1; NAP1; Neutrophil activating factor; Protein 3 10C
Species reactivity Human
Purity Natural and recombinant human IL-8
Shelf life Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Detection range 15.6 pg/ml - 1,000 pg/ml (cell culture supernates) 7.8 pg/ml - 500 pg/ml (serum, plasma)
Sample types
cell culture supernatantsserum and plasma (heparinEDTAcitrate).
See sample validation ›
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Sample Type Validation

4 sample types
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
cell culture supernatants
serum and plasma (heparin
EDTA
citrate).

Specifications

Target
Interleukin-8
Also known as
C X C motif chemokine 8; CXCL8; CXCL8; IL8; CXCL8/IL-8; Emoctakin; GCP 1; GCP1; IL 8; IL 8(1 77); IL8; IL-8; IL8/NAP1 form I Interleukin 8; IL8/NAP1 form II; IL8/NAP1 form VI; interleukin 8; MDNCF; MDNCF b IL 8(5 77) IL 8(6 77); NAF; NAF IL 8(7 77); NAP 1; NAP1; Neutrophil activating factor; Protein 3 10C
Purity
Natural and recombinant human IL-8
Detection range
15.6 pg/ml - 1,000 pg/ml (cell culture supernates) 7.8 pg/ml - 500 pg/ml (serum, plasma)
Sample types
cell culture supernatantsserum and plasma (heparinEDTAcitrate).
Species reactivity
Human

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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