ELISA Kit ABMIUM Verified™Collaborator Tested
ABM-33833

Human IL-12(p70) PicoKine® Quick ELISA Kit

ABMIUM Verified™|Collaborator Tested|Boster Bio

ELISA Kit supplied by Boster Bio Read more ›

Related formats
Human IL-12(p70) PicoKine® Quick ELISA Kit Boster Bio
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Human IL-12(p70) PicoKine® Quick ELISA Kit Boster Bio

Product Description

Human IL-12(P70) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate human IL12A in cell culture supernatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response.[i] And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation.[ii] Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70.[iii] The standard product used in this kit is recombinant human IL-12 p70 with the molecular mass of 75 KDa.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference FEK0421
Product category Quick ELISA Kits
Reactive species Human
Tested applications ELISA
Gene name IL12A
Protein name Interleukin-12 subunit alpha
Sample type cell culture supernatants and serum.
Assay range 7.8 pg/ml - 500 pg/ml
Sensitivity <2 pg/ml
Immunogen Expression system for standard: NS0; Immunogen sequence: (I23-S328) + (R23-S219)
Storage Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
UniProt P29459
NCBI Gene ID 3592

Available variants

Conjugation Size
Not applicable 96 wells/kit, with removable strips.

Key Facts

Product type ELISA Kit Target: Interleukin-12 subunit alpha
Target Interleukin-12 subunit alpha
Also known as CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL12; IL-12; IL12A; IL-12A; Interleukin 12 subunit alpha; NFSK; NKSF1; P35
Species reactivity Human
Shelf life Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Sensitivity <2 pg/ml
Detection range 7.8 pg/ml - 500 pg/ml
Sample types
cell culture supernatants and serum.
See sample validation ›
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
cell culture supernatants and serum.

Specifications

Target
Interleukin-12 subunit alpha
Also known as
CLMF; CLMF p35; IL 12 subunit p35; IL 12A; IL12; IL-12; IL12A; IL-12A; Interleukin 12 subunit alpha; NFSK; NKSF1; P35
Sensitivity
<2 pg/ml
Detection range
7.8 pg/ml - 500 pg/ml
Sample types
cell culture supernatants and serum.
Species reactivity
Human

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Long-term storage
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Storage conditions
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Shelf life
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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