Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human IGFBP3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IGFBP3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EZ0386
Product category	EZ-Set™ ELISA Kits (Ab Pairs)
Reactive species	Human
Tested applications	ELISA
Gene name	IGFBP3
Protein name	Insulin-like growth factor-binding protein 3
Sample type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay range	156 pg/ml - 10,000 pg/ml
Specificity	Natural and recombinant human IGFBP3
Immunogen	Expression system for standard: NS0; Immunogen sequence: A28Q-291
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P17936
NCBI Gene ID	3486

Available variants

Conjugation	Size
Not applicable	5 plates/kit