ABM-32670
Human HSP27/HSPB1/Heat Shock Protein 27 ELISA Kit PicoKine®
ABMIUM Verified™|Collaborator Tested|Boster Bio
ELISA Kit supplied by Boster Bio Read more ›
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Product Description
Human HSP27 / Heat Shock Protein 27 ELISA Kit PicoKine® (96 Tests). Quantitate Human HSPB1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, alpha-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kDa.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK0881 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Human |
| Tested applications | ELISA |
| Gene name | HSPB1 |
| Protein name | Heat shock protein beta-1 |
| Sample type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay range | 62.5 pg/ml-4000 pg/ml |
| Sensitivity | <5pg/ml |
| Specificity | Natural and recombinant human HSP27 |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: T2-K205 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P04792 |
| NCBI Gene ID | 3315 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
Product type
ELISA Kit›
Target: Heat shock protein beta-1
| Target | Heat shock protein beta-1 |
| Also known as | Heat shock protein beta 1; HSP 27; Hsp25; HSP27; HSP28; HSPB1 |
| Species reactivity | Human |
| Purity | Natural and recombinant human HSP27 |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <5pg/ml |
| Detection range | 62.5 pg/ml-4000 pg/ml |
| Sample types |
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
See sample validation ›
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Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| cell lysates | |||||
| serum and plasma (heparin | |||||
| EDTA). |
Specifications
Target
Heat shock protein beta-1
Also known as
Heat shock protein beta 1; HSP 27; Hsp25; HSP27; HSP28; HSPB1
Purity
Natural and recombinant human HSP27
Sensitivity
<5pg/ml
Detection range
62.5 pg/ml-4000 pg/ml
Sample types
cell culture supernatantscell lysatesserum and plasma (heparinEDTA).
Species reactivity
Human
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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