ABM-33627
Human GIF ELISA Kit PicoKine®
ABMIUM Verified™|Collaborator Tested|Boster Bio
ELISA Kit supplied by Boster Bio Read more ›
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Product Description
Human GIF ELISA Kit PicoKine® (96 Tests). Quantitate Human CBLIF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Intrinsic factor (IF), also known as gastric intrinsic factor (GIF), is a glycoprotein produced by the parietal cells of the stomach. It is necessary for the absorption of vitamin B12 later on in the ileum of the small intestine. In humans, the gastric intrinsic factor protein is encoded by the GIF gene. It is mapped to 11q12.1. Haptocorrin (also known as HC, R protein, and transcobalamin I, TCN1) is another glycoprotein secreted by the salivary glands which binds to vitamin B12. Vitamin B12 is acid sensitive and in binding to transcobalamin I it can safely pass through the acidic stomach to the duodenum. In the less acidic environment of the small intestine, pancreatic enzymes digest the glycoprotein carrier and vitamin B12 can then bind to intrinsic factor
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | EK1952 |
| Product category | PicoKine® ELISA Kits |
| Reactive species | Human |
| Tested applications | ELISA |
| Gene name | CBLIF |
| Protein name | Cobalamin binding intrinsic factor |
| Sample type | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
| Assay range | 15.6 pg/ml - 1,000 pg/ml |
| Sensitivity | <10 pg/ml |
| Specificity | Natural and recombinant human GIF |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: S19-Y417 |
| Storage | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| UniProt | P27352 |
| NCBI Gene ID | 2694 |
Available variants
| Conjugation | Size |
|---|---|
| Not applicable | 96 wells/kit, with removable strips. |
Key Facts
Product type
ELISA Kit›
Target: Cobalamin binding intrinsic factor
| Target | Cobalamin binding intrinsic factor |
| Also known as | CBLIF; GIF; IFMH; Intrinsic factor; IF; INF; TCN3 |
| Species reactivity | Human |
| Purity | Natural and recombinant human GIF |
| Shelf life | Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.) |
| Sensitivity | <10 pg/ml |
| Detection range | 15.6 pg/ml - 1,000 pg/ml |
| Sample types |
cell culture supernatantsserum and plasma (heparinEDTAcitrate).
See sample validation ›
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Sample Type Validation
4 sample types| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| cell culture supernatants | |||||
| serum and plasma (heparin | |||||
| EDTA | |||||
| citrate). |
Specifications
Target
Cobalamin binding intrinsic factor
Also known as
CBLIF; GIF; IFMH; Intrinsic factor; IF; INF; TCN3
Purity
Natural and recombinant human GIF
Sensitivity
<10 pg/ml
Detection range
15.6 pg/ml - 1,000 pg/ml
Sample types
cell culture supernatantsserum and plasma (heparinEDTAcitrate).
Species reactivity
Human
Storage & Stability
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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