Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human Fibronectin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FN1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EZ0349
Product category	EZ-Set™ ELISA Kits (Ab Pairs)
Reactive species	Human
Tested applications	ELISA
Gene name	FN1
Protein name	Fibronectin
Sample type	cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Assay range	156 pg/ml - 10,000 pg/ml
Specificity	Natural and recombinant human Fibronectin beta
Immunogen	Expression system for standard: from plasma
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P02751
NCBI Gene ID	2335

Available variants

Conjugation	Size
Not applicable	5 plates/kit