Human CDNF ELISA Kit PicoKine®

Human CDNF ELISA Kit PicoKine® (96 Tests). Quantitate Human CDNF in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Cerebral dopamine neurotrophic factor also known as ARMET-like protein 1 or is a protein that in humans that is encoded by the CDNF gene. CDNF and MANF are secreted proteins with 8 conserved cysteine residues, which predict a unique protein fold and define an evolutionarily conserved protein family. The human CDNF mRNA encodes a 187-amino acid protein with a predicted signal peptide and apparently no prosequence. The predicted secondary structure, like that of MANF, is dominated by alpha-helices. Human CDNF shares 59% amino acid identity with human MANF, and 49% and 46% identity with D. melanogaster and C. elegans Manf proteins, respectively. RT-PCR of mouse brain detected Cdnf transcripts in embryonic and postnatal stages, and in adult brain regions, including the striatum. In the midbrain, Cdnf mRNA was expressed from embryonic stages to the adult. High levels of Cdnf mRNA were observed in adult mouse heart, skeletal muscle, and testis. In agreement with the results from mouse, wide expression of CDNF transcripts was detected in human brain and nonneuronal tissues. Interestingly, levels of CDNF transcripts were relatively high in the corpus callosum and optic nerve, which are devoid of neuronal cell bodies and contain mostly axonal projections and oligodendrocytes. At postnatal stage P10 of the mouse brain, Cdnf staining was most intense in the hippocampus and thalamus. Cdnf signal was also detected in striatum and substantia nigra.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK1656
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	CDNF
Protein name	Cerebral dopamine neurotrophic factor
Sample type	cell culture supernatants, serum and plasma (heparin, EDTA).
Assay range	15.6 pg/ml - 1,000 pg/ml
Sensitivity	<10 pg/ml
Specificity	Natural and recombinant human CDNF
Immunogen	Expression system for standard: E.coli; Immunogen sequence: Q25-L187
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	Q49AH0
NCBI Gene ID	441549

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.