Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

Human ADA/Adenosine Deaminase ELISA Kit PicoKine® (96 Tests). Quantitate Human ADA in cell culture supernatants, serum and pleural fluid. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK1446
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	ADA
Protein name	Adenosine deaminase
Sample type	cell culture supernatants, serum and pleural fluid.
Assay range	31.2 pg/ml - 2,000 pg/ml
Sensitivity	<10 pg/ml
Specificity	Natural and recombinant human ADA
Immunogen	Expression system for standard: NS0; Immunogen sequence: A2-L363
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P00813
NCBI Gene ID	100

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.