Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine®

Human A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine® (96 Tests). Quantitate Human A2M in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Product information

Supplier	Boster Bio
ABMIUM evidence status	ABMIUM Verified™ collaborator-tested data
Catalogue reference	EK1118
Product category	PicoKine® ELISA Kits
Reactive species	Human
Tested applications	ELISA
Gene name	A2M
Protein name	Alpha-2-macroglobulin
Sample type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay range	625 pg/ml - 40,000 pg/ml
Sensitivity	<20 pg/ml
Specificity	Natural human A2M
Immunogen	Target protein isolated from human plasma
Storage	Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
UniProt	P01023
NCBI Gene ID	2

Available variants

Conjugation	Size
Not applicable	96 wells/kit, with removable strips.