Collaborator Boster Bio
Secondary AntibodiesABMIUM Verified™Collaborator Tested
ABM-33952

HRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L)

ABMIUM Verified™|Collaborator Tested|Boster Bio

Secondary Antibody supplied by Boster Bio Read more ›

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Product Description

HRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) is a research-use antibody from Boster Bio for studying HRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) in ELISA, WB workflows with listed reactivity for Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

HRP Conjugated Rabbit Anti-rat IgG (H+L) (gamma-chain specific) secondary antibody This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG.

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference BA1058
Product category Secondary Antibodies
Reactive species Rat
Tested applications ELISA, WB
Available conjugations HRP
Specificity This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG.
Immunogen Rat IgG (whole molecule).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Purification The antibody was purified from antisera by immunoaffinity chromatography.
Storage At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
NCBI Gene ID 6494

Available variants

Conjugation Size
HRP 0.5ml
HRP 1ml

Key Facts

Product typeSecondary Antibodies
Collaborator Boster Bio
Partner model BA1058
Reactivity Rat
Applications ELISA,WB
ConjugationHRP
Dilution rangeWestern Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Rat IgG (whole molecule).
Concentration 1mg/ml
Purification The antibody was purified from antisera by immunoaffinity chromatography.
Purity This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG.
Shelf life At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Rat

Specifications

Concentration
1mg/ml
Purity
This HRP conjugated antibody is specific for rat IgG and shows no cross-reactivity with human/bovine/goat/rabbit IgG.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
HRP
Species reactivity
Rat
Tested applications
ELISAWB
Immunogen
Rat IgG (whole molecule).
Dilution range
Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)
Purification
The antibody was purified from antisera by immunoaffinity chromatography.

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
Storage buffer
0.5 mg of HRP conjugated specific antibody, 0.01M PBS (pH7.4), 50% glycerol.
Shelf life
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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