HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Antibody supplied by Boster Bio Read more ›

Product Description
Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | BA1054 |
| Product category | Secondary Antibodies |
| Reactive species | Rabbit |
| Tested applications | ELISA, WB |
| Available conjugations | HRP |
| Specificity | This HRP conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG. |
| Immunogen | Rabbit IgG (whole molecule). |
| Host | Goat |
| Clonality | Polyclonal |
| Purification | The antibody was purified from antisera by immunoaffinity chromatography. |
| Storage | At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. |
| NCBI Gene ID | 338167 |
Available variants
| Conjugation | Size |
|---|---|
| HRP | 0.5ml |
| HRP | 1ml |
Key Facts
| Concentration | 1mg/ml |
| Species reactivity | Rabbit |
| Purity | This HRP conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG. |
| Shelf life | At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. |
| Host species | Goat |
| Clonality | Polyclonal |
| Conjugation | HRP |
| Tested applications |
ELISAWB |
| Dilution range | Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection) |
| Immunogen | Rabbit IgG (whole molecule). |
| Purification | The antibody was purified from antisera by immunoaffinity chromatography. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Rabbit |
Specifications
Western Blotting: 0.7-3.3μg/ml (DAB detection)
ELISA: 0.05-0.5μg/ml (TMB detection)
Storage & Stability
Compliance & Certifications
Not intended for diagnostic or therapeutic use.