Secondary Antibody ABMIUM Verified™Collaborator Tested
ABM-33957

HRP Conjugated AffiniPure Goat Anti-Human IgM

ABMIUM Verified™|Collaborator Tested|Boster Bio

Secondary Antibody supplied by Boster Bio Read more ›

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Product Description

HRP Conjugated Goat Anti-human IgM (u-chain specific) secondary antibody This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference BA1077
Product category Secondary Antibodies
Reactive species Human
Tested applications ELISA, WB
Available conjugations HRP
Specificity This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.
Immunogen Human IgM (whole molecule).
Host Goat
Clonality Polyclonal
Isotype IgM
Purification The antibody was purified from antisera by immunoaffinity chromatography.
Storage At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
NCBI Gene ID 6494

Available variants

Conjugation Size
HRP 0.25ml
HRP 0.5ml

Key Facts

Product type Secondary Antibody
Concentration 1mg/ml
Species reactivity Human
Purity This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.
Form / buffer Concentrated, Liquid
Shelf life At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
Host species Goat
Clonality Polyclonal
Isotype IgM
Conjugation HRP
Tested applications
ELISAWB
Dilution range Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)
Immunogen Human IgM (whole molecule).
Purification The antibody was purified from antisera by immunoaffinity chromatography.
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Reactivity & Application Validation

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Human

Specifications

Concentration
1mg/ml
Purity
This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.
Form / buffer
Concentrated, Liquid
Host species
Goat
Clonality
Polyclonal
Isotype
IgM
Conjugation
HRP
Species reactivity
Human
Tested applications
ELISAWB
Immunogen
Human IgM (whole molecule).
Dilution range
Western Blotting: 0.1-0.2μg/ml (ECL detection) Western Blotting: 0.7-3.3μg/ml (DAB detection) ELISA: 0.05-0.5μg/ml (TMB detection)
Purification
The antibody was purified from antisera by immunoaffinity chromatography.

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
Long-term storage
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
Storage conditions
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.
Storage buffer
0.25 mg of HRP conjugated specific antibody, 0.01 M PBS (pH7.4), 50% glycerol.
Shelf life
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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