Secondary Antibody supplied by Boster Bio Read more ›
Goat Anti-Rabbit IgG (H+L) Secondary Antibody is a research-use antibody from Boster Bio for studying Goat Anti-Rabbit IgG (H+L) Secondary Antibody in Flow Cytometry, IF/ICC workflows with listed reactivity for Rabbit. Specifications and supporting documents below should be reviewed when planning the experiment.
This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | BA1142 |
| Product category | Secondary Antibodies |
| Reactive species | Rabbit |
| Tested applications | Flow Cytometry, IF/ICC |
| Available conjugations | Fluoro594 |
| Specificity | This Fluoro594 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG. |
| Immunogen | Rabbit IgG (whole molecule). |
| Host | Goat |
| Clonality | Polyclonal |
| Storage | At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
| NCBI Gene ID | 337843 |
| Conjugation | Size |
|---|---|
| Fluoro594 | 0.5ml |
| Fluoro594 | 1ml |
| Collaborator | Boster Bio |
| Partner model | BA1142 |
| Reactivity | Rabbit |
| Applications | Flow Cytometry,IF/ICC |
| Conjugation | Fluoro594 |
| Dilution range | Immunofluorescence: 2-10 μg/mL<br> Flow Cytometry (Fixed): 2-10 μg/mL |
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Rabbit IgG (whole molecule). |
| Concentration | 1mg/ml |
| Purity | This Fluoro594 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG. |
| Shelf life | At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Rabbit |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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