Secondary Antibody ABMIUM Verified™Collaborator Tested
ABM-33913

Goat Anti-Rabbit IgG (H+L) Secondary Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Secondary Antibody supplied by Boster Bio Read more ›

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Product Description

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference BA1142
Product category Secondary Antibodies
Reactive species Rabbit
Tested applications Flow Cytometry, IF/ICC
Available conjugations Fluoro594
Specificity This Fluoro594 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.
Immunogen Rabbit IgG (whole molecule).
Host Goat
Clonality Polyclonal
Storage At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
NCBI Gene ID 337843

Available variants

Conjugation Size
Fluoro594 0.5ml
Fluoro594 1ml

Key Facts

Product type Secondary Antibody
Concentration 1mg/ml
Species reactivity Rabbit
Purity This Fluoro594 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.
Shelf life At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Host species Goat
Clonality Polyclonal
Conjugation Fluoro594
Tested applications
Flow CytometryIF/ICC
Dilution range Immunofluorescence: 2-10 μg/mL
Flow Cytometry (Fixed): 2-10 μg/mL
Immunogen Rabbit IgG (whole molecule).
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Reactivity & Application Validation

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Rabbit

Specifications

Concentration
1mg/ml
Purity
This Fluoro594 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.
Host species
Goat
Clonality
Polyclonal
Conjugation
Fluoro594
Species reactivity
Rabbit
Tested applications
Flow CytometryIF/ICC
Immunogen
Rabbit IgG (whole molecule).
Dilution range
Immunofluorescence: 2-10 μg/mL
Flow Cytometry (Fixed): 2-10 μg/mL

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Long-term storage
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Storage conditions
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Shelf life
At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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