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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM100546

NGDN rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NGDN rabbit pAb from ELK Biotechnology Read more ›

NGDN rabbit pAb
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NGDN rabbit pAb

Product Description

NGDN rabbit pAb is a research-use antibody from ELK Biotechnology for studying NGDN in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Neuroguidin is an EIF4E (MIM 133440)-binding protein that interacts with CPEB (MIM 607342) and functions as a translational regulatory protein during development of the vertebrate nervous system (Jung et al., 2006 [PubMed 16705177]).[supplied by OMIM, Mar 2008],

Additional antibody information

Specificity NGDN Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: NGDN
Collaborator ELK Biotechnology
Partner model ES9910
Target NGDN
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 240-320
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Epithelium,Placenta,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000 —
Mouse — —
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
NGDN
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 240-320
Observed band
34kD
Cellular localization
Nucleus . Nucleus, nucleolus . Chromosome, centromere . Cytoplasm . Cell projection, axon . Cell projection, dendrite . Cell projection, filopodium . Detected in axons, dendrites and filopodia. Colocalized with EIF4E in neurites (By similarity). Translocated from nucleolus to nuclear foci in response to UV damage. .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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