Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM100417

GBB3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GBB3 rabbit pAb from ELK Biotechnology Read more ›

GBB3 rabbit pAb
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GBB3 rabbit pAb

Product Description

GBB3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GBB3 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced tr

Additional antibody information

Specificity GBB3 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: GBB3
Collaborator ELK Biotechnology
Partner model ES9701
Target GBB3
Research areas>>Ras signaling pathway, >>Chemokine signaling pathway, >>PI3K-Akt signaling pathway, >>Apelin signaling pathway, >>Circadian entrainment, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>GABAergic synapse, >>Dopaminergic synapse, >>Taste transduction, >>Relaxin signaling pathway, >>Morphine addiction, >>Alcoholism, >>Human cytomegalovirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Colon carcinoma,Eye,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GBB3
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
37kD
Cellular localization
cytosol,plasma membrane,dendrite,cell body,extracellular exosome,
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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