ABM99985
CLUS rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CLUS rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CLUS rabbit pAb is an ELK Biotechnology antibody for research workflows involving CLUS.
Background
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011],
Additional antibody information
| Specificity | This antibody detects endogenous levels of CLUS. |
|---|---|
| Validation evidence captions | Western blot analysis of MCF-7 lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
Product type
Primary Antibody›
Target: CLUS
| Target | CLUS |
| Also known as | Clusterin (Aging-associated gene 4 protein) (Apolipoprotein J) (Apo-J) (Complement cytolysis inhibitor) (CLI) (Complement-associated protein SP-40,40) (Ku70-binding protein 1) (NA1/NA2) (Testosterone-repressed prostate message 2) (TRPM-2) Cleaved into: Clusterin beta chain (ApoJalpha) (Complement cytolysis inhibitor a chain); Clusterin alpha chain (ApoJbeta) (Complement cytolysis inhibitor b chain) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000, ELISA 1:10000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 52kD |
| Cellular localization | Isoform 1: Secreted . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. .; Isoform 4: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; Isoform 6: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; Nucleus . Cytoplasm . Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Mitochondrion . Mitochondrion membrane . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress (PubMed:17451556). Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Complement and coagulation cascades |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000, ELISA 1:10000-20000 |
| Immunogen | Synthesized peptide derived from human CLUS Polyclonal |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
CLUS
Also known as
Clusterin (Aging-associated gene 4 protein) (Apolipoprotein J) (Apo-J) (Complement cytolysis inhibitor) (CLI) (Complement-associated protein SP-40,40) (Ku70-binding protein 1) (NA1/NA2) (Testosterone-repressed prostate message 2) (TRPM-2) Cleaved into: Clusterin beta chain (ApoJalpha) (Complement cytolysis inhibitor a chain); Clusterin alpha chain (ApoJbeta) (Complement cytolysis inhibitor b chain)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human CLUS Polyclonal
Observed band
52kD
Cellular localization
Isoform 1: Secreted . Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. .; Isoform 4: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; Isoform 6: Cytoplasm . Keeps cytoplasmic localization in stressed and unstressed cell. .; Nucleus . Cytoplasm . Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side . Cytoplasm, cytosol . Microsome . Endoplasmic reticulum . Mitochondrion . Mitochondrion membrane . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, chromaffin granule . Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress (PubMed:17451556). Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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