Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99953

IGF1R (Phospho-Tyr1161) Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IGF1R (Phospho-Tyr1161) Rabbit pAb from ELK Biotechnology Read more ›

IGF1R (Phospho-Tyr1161) Rabbit pAb
Zoom
IGF1R (Phospho-Tyr1161) Rabbit pAb

Product Description

IGF1R (Phospho-Tyr1161) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying IGF1R in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014],

Additional antibody information

Specificity This detects endogenous levels of IGF1R (Phospho-Tyr1161)
Validation evidence captions Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: IGF1R
Collaborator ELK Biotechnology
Partner model ES8878
Target IGF1R
Research areas>>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Oocyte meiosis, >>Autophagy - animal, >>Endocytosis, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Focal adhesion, >>Adherens junction, >>Signaling pathways regulating pluripotency of stem cells, >>Long-term depression, >>Ovarian steroidogenesis, >>Progesterone-mediated oocyte maturation, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Proteoglycans in cancer, >>Glioma, >>Prostate cancer, >>Melanoma, >>Breast cancer, >>Hepatocellular carcinoma
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phospho derived from human IGF1R (Phospho-Tyr1161)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IGF1R
Also known as
Insulin-like growth factor 1 receptor (EC 2.7.10.1) (Insulin-like growth factor I receptor) (IGF-I receptor) (CD antigen CD221) Cleaved into: Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized phospho derived from human IGF1R (Phospho-Tyr1161)
Observed band
pro: 155kD, recetor beta: 95kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein .
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.