Smad3 (Phospho-Ser213) Rabbit pAb from ELK Biotechnology Read more ›
Smad3 (Phospho-Ser213) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Smad3 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],
| Specificity | This detects endogenous levels of Smad3 (Phospho-Ser213) |
|---|---|
| Validation evidence captions | Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES8877 |
| Target | Smad3 |
| Research areas | >>FoxO signaling pathway, >>Cell cycle, >>Endocytosis, >>Cellular senescence, >>Wnt signaling pathway, >>TGF-beta signaling pathway, >>Apelin signaling pathway, >>Hippo signaling pathway, >>Adherens junction, >>Signaling pathways regulating pluripotency of stem cells, >>Th17 cell differentiation, >>AGE-RAGE signaling pathway in diabetic complications, >>Hepatitis B, >>Human T-cell leukemia virus 1 infection, >>Pathways in cancer, >>Colorectal cancer, >>Pancreatic cancer, >>Chronic myeloid leukemia, >>Hepatocellular carcinoma, >>Gastric cancer, >>Inflammatory bowel disease, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho derived from human Smad3 (Phospho-Ser213) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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