PARP-1 (Acetyl-K521) rabbit pAb
PARP-1 (Acetyl-K521) rabbit pAb from ELK Biotechnology Read more ›
Product Description
PARP-1 (Acetyl-K521) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PARP.
Background
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of PARP-1 at Human:K521;Mouse:K520;Rat:K521, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 1,mouse-heart 2,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) | Western Blot analysis of 1,293t 2,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) |
Key Facts
| Target | PARP |
| Also known as | Poly ADP-ribose polymerase 1 (PARP-1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 1) (ARTD1) (NAD(+) ADP-ribosyltransferase 1) (ADPRT 1) (PolyADP-ribose synthase 1) |
| Concentration | 1 mg/ml |
| Working concentration | wb dilution 1:1000 |
| Species reactivity | Human;Mouse;Rat |
| Cellular localization | Nucleus . Nucleus, nucleolus . Chromosome . Localizes to sites of DNA damage. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Base excision repair;>>NF-kappa B signaling pathway;>>Apoptosis;>>Necroptosis;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | wb dilution 1:1000 |
| Immunogen | Synthesized Acetyl peptide derived from human PARP-1. at AA range: K521 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | wb dilution 1:1000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.