ACSS1 (Acetyl-K642) rabbit pAb from ELK Biotechnology Read more ›
ACSS1 (Acetyl-K642) rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACSS1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],
| Specificity | This antibody detects endogenous levels of ACSS1 at Human:K642;Mouse:K635;Rat:K635, It doesn't reacte with total protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 1,293T 2,hela cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) |
| Collaborator | ELK Biotechnology |
| Partner model | ES8818 |
| Target | ACSS1 |
| Research areas | >>Glycolysis / Gluconeogenesis, >>Pyruvate metabolism, >>Glyoxylate and dicarboxylate metabolism, >>Propanoate metabolism, >>Metabolic pathways, >>Carbon metabolism |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized Acetyl peptide derived from human ACSS1. at AA range: K642 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Amygdala,Brain,PCR rescued clones,Placenta,Spleen,Stomach,T |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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