Cdk6 rabbit pAb from ELK Biotechnology Read more ›
Cdk6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cdk6 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
cyclin dependent kinase 6(CDK6) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefS
| Specificity | The antibody detects endogenous Cdk6 |
|---|---|
| Validation evidence captions | Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8658 |
| Target | Cdk6 |
| Research areas | >>Cell cycle, >>p53 signaling pathway, >>PI3K-Akt signaling pathway, >>Cellular senescence, >>Cushing syndrome, >>Hepatitis C, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Viral carcinogenesis, >>MicroRNAs in cancer, >>Pancreatic cancer, >>Glioma, >>Melanoma, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Breast cancer, >>Hepatocellular carcinoma |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide from human protein at AA range: 280-325 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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