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Primary Antibody ISO 9001|ABMIUM Verified™
ABM99899

Cdk6 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Cdk6 rabbit pAb from ELK Biotechnology Read more ›

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Cdk6 rabbit pAb
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Cdk6 rabbit pAb

Product Description

Cdk6 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cdk6.

Background

cyclin dependent kinase 6(CDK6) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Expression of this gene is up-regulated in some types of cancer. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefS

Additional antibody information

Specificity The antibody detects endogenous Cdk6
Validation evidence captions Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200

Key Facts

Product type Primary Antibody› Target: Cdk6
Target Cdk6
Also known as Cyclin-dependent kinase 6 (EC 2.7.11.22;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE)
Concentration 1 mg/ml
Working concentration WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
Species reactivity Human;Rat;Mouse;
Observed band 34kD
Cellular localization Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663). .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area >>Cell cycle;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Measles;>>Human cytomegalovirus infection;>>Influenza A;>>Human papillomavirus infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>MicroRNAs in cancer;>>Pancreatic cancer;>>Glioma;>>Melanoma;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHCIFELISA
Dilution range WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
Immunogen Synthetic peptide from human protein at AA range: 280-325
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 —
Rat — —
Mouse — —
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Cdk6
Also known as
Cyclin-dependent kinase 6 (EC 2.7.11.22;Cell division protein kinase 6;Serine/threonine-protein kinase PLSTIRE)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
Synthetic peptide from human protein at AA range: 280-325
Observed band
34kD
Cellular localization
Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663). .
Dilution range
WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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