EGF rabbit pAb
EGF rabbit pAb from ELK Biotechnology Read more ›
Product Description
EGF rabbit pAb is an ELK Biotechnology antibody for research workflows involving EGF.
Background
This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016],
Additional antibody information
| Specificity | The antibody detects endogenous EGF |
|---|---|
| Validation evidence captions | Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200 |
Key Facts
| Target | EGF |
| Also known as | Pro-epidermal growth factor (EGF) Cleaved into: Epidermal growth factor (Urogastrone) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 130kD |
| Cellular localization | Membrane; Single-pass type I membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phospholipase D signaling pathway;>>PI3K-Akt signaling pathway;>>Focal adhesion;>>Gap junction;>>JAK-STAT signaling pathway;>>Regulation of actin cytoskeleton;>>Hepatitis C;>>Human papillomavirus infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Chemical carcinogenesis - reactive oxygen species;>>Colorectal cancer;>>Pancreatic cancer;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Gastric cancer;>>Choline metabolism in cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000 |
| Immunogen | Synthetic peptide from human protein at AA range: 1000-1060 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.