Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Rabbit pAb from ELK Biotechnology Read more ›
Sodium Potassium ATPase alpha-1 (Phospho-Tyr260) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Na+/K+-ATPase α1 in WB, IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
| Specificity | The antibody detects endogenous Sodium Potassium ATPase alpha-1 when Phospho occurs at Tyr260) |
|---|---|
| Validation evidence captions | Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Na+/K+-ATPase α1 (Phospho-Tyr260) Antibody | Western blot analysis of lysates from 293 cells treated with PMA, using phospho-Na+/K+-ATPase α1 (Phospho-Tyr260) antibody. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES8616 |
| Target | Na+/K+-ATPase α1 |
| Research areas | >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Cardiac muscle contraction, >>Adrenergic signaling in cardiomyocytes, >>Insulin secretion, >>Thyroid hormone synthesis, >>Thyroid hormone signaling pathway, >>Aldosterone synthesis and secretion, >>Aldosterone-regulated sodium reabsorption, >>Endocrine and other factor-regulated calcium reabsorption, >>Proximal tubule bicarbonate reclamation, >>Salivary secretion, >>Gastric acid secretion, >>Pancreatic secretion, >>Carbohydrate digestion and absorption, >>Protein digestion and absorption, >>Bile secretion, >>Mineral absorption |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide from human protein at AA range: 230-290 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Cerebellum,Cervix,Placenta,Retinal pigment epithelium |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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