Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99875

IL16 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IL16 rabbit pAb from ELK Biotechnology Read more ›

IL16 rabbit pAb
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IL16 rabbit pAb

Product Description

IL16 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL16 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010],

Additional antibody information

Specificity The antibody detects endogenous IL16
Validation evidence captions Western blot analysis of MCF7 SW480 MOUSE-LIVER mouse-lung MOUSE-KIDNEY lysate, antibody was diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000

Key Facts

Product typePrimary Antibody Target: IL16
Collaborator ELK Biotechnology
Partner model ES8610
Target IL16
Research areas>>Cytokine-cytokine receptor interaction
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000, ELISA 1:10000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic peptide from human protein at AA range: 900-960
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Isoform 3: Expressed in hemopoietic tissues, such as resting T-cells, but undetectable during active T-cell proliferation.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IL16
Also known as
Pro-interleukin-16 Cleaved into: Interleukin-16 (IL-16) (Lymphocyte chemoattractant factor) (LCF)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthetic peptide from human protein at AA range: 900-960
Observed band
67kD
Cellular localization
Interleukin-16: Secreted.; Isoform 1: Cytoplasm .; Isoform 3: Cytoplasm. Nucleus.
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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