GRP78 BiP rabbit pAb from ELK Biotechnology Read more ›
GRP78 BiP rabbit pAb is a research-use antibody from ELK Biotechnology for studying HSP A5/GRP78 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Fish. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010],
| Specificity | The antibody detects endogenous GRP78 BiP |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES8607 |
| Target | HSP A5/GRP78 |
| Research areas | >>Protein export, >>Protein processing in endoplasmic reticulum, >>Antigen processing and presentation, >>Thyroid hormone synthesis, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat,Fish |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000.IHC-p/f:1:50-300. ELISA 1:10000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide from human protein at AA range: 505-570 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Articular cartilage,Brain,Cajal-Retzius cell,Cervix carcino |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Fish |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000.IHC-p/f:1:50-300. ELISA 1:10000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Fish | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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