HLA Class I rabbit pAb from ELK Biotechnology Read more ›
HLA Class I rabbit pAb is a research-use antibody from ELK Biotechnology for studying HLA Class I in WB, ELISA workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney trans
| Specificity | The antibody detects endogenous HLA Class I protein |
|---|---|
| Validation evidence captions | Western Blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysates from Ramos cells, using HLA Class I antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES8555 |
| Target | HLA Class I |
| Research areas | Antibody Research |
| Reactivity | Human |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000, ELISA 1:10000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HLA Class I. AA range:204-253 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | A*3201,Blood,Brain,Buffy coat,Hematopoietic,Liver,Lung,Lymp |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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